Application

Western Blotting Analysis: A representative lot detected Cdh1 in Western Blotting applications (Garzon, J., et. al. (2017). Oncogene. 36(42):5808-5818).

Anti-Cdh1, clone AR38, Cat. No. MABT1323, is a mouse monoclonal antibody that detects Cdh1/FZR1 and has been tested for use in Western Blotting.

General description

Fizzy-related protein homolog (UniProt: Q9R1K5; also known as Fzr, Cdh1/Hct1 homolog) is encoded by the Fzr1 (also known as Fyr, Fzr) gene (Gene ID: 56371) in murine species. Cdh1 is a substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex that associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. APC/C is activated upon sequential binding to the Cdc20 and Cdh1 adaptor proteins. Cdc20 associates with APC/C during early mitosis and principally regulates mitotic progression, while Cdh1 interacts with the APC/C from late mitosis onwards until the following G1/S transition. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition Cdh1 is phosphorylated, leading to its dissociation from the APC/C. It has been shown that Cdk-mediated Cdh1 phosphorylation prevents Cdh1 from associating with APC/C prematurely during S/G2 phase, while CDC14B phosphatase-dependent APC/C-Cdh1 activation in response to DNA damage during G2 phase leads to ubiquitination and degradation of Polo-like kinase 1 (Plk1) and prevents entry into mitosis. Following DNA repair, Cdk1 and Plk1 are reactivated and cell cycle progresses from G2 into mitosis phase.

Immunogen

His-tagged full-length recombinant mouse Fizzy-related protein homolog (Cdh1).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Cdh1 in HeLa cell lysate.

Specificity

Clone AR38.2 detects Cdh1 (Fizzy-related protein homolog) in human and mouse cells.

Target description

~54 kDa observed; 54.69 kDa calculated. Uncharacterized bands may be observed in some lysate(s).